Antibodies and complement : Effector functions, therapies and technologies
28 June – 01 July 2018 | Girona, Spain
About the workshop
The antibody response and the complement system are major effector arms of the immune system. Both have been involved in a plethora of pathological conditions—including autoimmunity, allergy, infections and cancer—and regulate, mainly through their receptors (FcR and CR respectively) cell activation, migration, survival but also metabolism. As the portfolio of antibody—and complement-effector function grow, high-end technological solutions are developed and novel therapeutic applications are proposed.
This EMBO Workshop will focus attention on key advances in both fields with the aim at fostering discussions between antibody experts and complement experts. Some of the critical topics to be covered include antibody & complement functions, FcR and CR, Ab glycosylation and anti-inflammatory activity, Structure-function linkage, Novel technologies, complement and antibody therapies.
This EMBO Workshop on Antibody & Complement will be a forum for leaders in the field and new investigators coming from academia, industry and research institutes to discuss where the field is going and what challenges lie ahead.
Image credits: Joost Bakker (Scicomvisuals, Amsterdam) and https://www.ncbi.nlm.nih.gov/pubmed/24626930
16:10-16:20 Welcome & Introductions
Pierre Bruhns & Falk Nimmerjahn
1: NOVEL Ab FUNCTIONS
16:20-16:55 IgG1 is an inhibitory IgG subclass
16:55-17:10 Oral presentation 10+5’: Testing the protective effect of monoclonal anti-influenza B neuraminidase IgG, IgM and IgA antibodies in a murine challenge model
17:10-17:45 Human IgG2 agonists – Big doors swing on little hinges
17:45-18:00 Oral presentation 10+5’: HIV-1 envelope specific IgA triggers antibody-dependent cell cytotoxicity and cooperates with IgG to increase cell lysis
18:00-18:35 Trim-Away: Targeted degradation of pathogens and proteins by the cytosolic antibody receptor TRIM21
18:45 Welcome Reception – Networking
(with food & drinks)
Poster Session I
20:00-22:30 Poster Session I: # 10 (Marshall), 11 (Eyer), 14 (Chromikova), 15 (Krištić), 20 (Zaytseva) 21 (Lux), 25 (Merchand Reyes), 26 (Ganesan), 28 (Morley), 32 (Mané Damas) 40 (Conde), 43 (Wang), 48 (Sopp), 50 (Biburger), 51 (Mutti), 59 (Liu) , 63 (Losen) 72 (Castro Dopico), 73 (Strasser), 75 (Deveuve), 79 (Prasad)
2: NOVEL COMPLEMENT FUNCTIONS
09:00-09:35 Novel roles of complement proteins uncover opportunities for neurotherapeutics
09:35-09:50 Oral presentation 10+5’: Complement activation by anti-HIV-1 broadly neutralizing antibodies
09:50-10:05 Oral presentation 10+5’: C5a regulates natural antibody response and egress of peritoneal B1 cells
10:05-10:40 Dissecting FcR-dependent mechanisms in FcR-humanized mice
10:40-11:15 Coffee Break
3: STRUCTURAL CONCEPTS
11:15-11:50 Sweet treasures from discarded human tissue: natural killer cells specifically modify CD16a to bind antibody tightly
11:50-12:05 Oral presentation 10+5’: A Revised structure of IgM pentamer, an asymmetric pentagon with an open groove that AIM protein fits in.
12:05-12:20 Oral presentation 10+5’: Molecular dissection of the interaction between FcγRIIIa and an allosteric Affimer inhibitor
12:20-12:55 Molecular recognition of IgG-Fc by Fcgamma receptors
Jose MM Caaveiro
13:00-16:20 LUNCH and free time
16:20-16:55 Systems serology
16:55-17:10 Oral presentation 10+5’: Key immunomodulatory role of neutrophils in the induction of protective immunity by antiviral monoclonal antibodies
17:10-17:25 Oral presentation 10+5’: Neutrophils and alveolar macrophages crosstalk during immune complex-mediated acute airway inflammation
17:25-18:20 Molecular mechanisms of complement activation by IgG
Poster Session II
18:30-20:00 Poster Session II: # 4 (Thomann), 7 (Natali), 9 (Stach), 18 (Foy), 22 (Pelegrin), 23 (Buteyn) 27 (Polezhaeva), 29 (Miyasaki), 30 (Macari), 31 (Bakshi), 36 (Lankipalli), 38 (Strumane) 46 (Godon), 52 (Ipsen Escobedo), 54 (Schlothauer), 57 (Fernández), 58 (Mottet) 66 (Sterlin), 70 (Lee), 71 (Delidakis), 77 (Zoldos)
09:00-09:35 Regulation of autoantibody activity via glycosylation
09:35-09:50 Oral presentation 10+5’: Investigation of Fc Receptor Binding and Pharmacokinetics of Distinct Fc Glycans
09:50-10:05 Oral presentation 10+5’: Restricted processing of CD16a/Fc γ receptor IIIa N-glycans from primary human NK cells impacts structure and function
10:05-10:40 The role of the IL23/Th17 axis in the control of antibody activity and autoimmune-mediated inflammatory disease
10:40-11:15 Coffee Break
6: REGULATING Ab & COMPLEMENT ACTIVITY
11:15-11:50 Novel antibodies and a new mechanism of diversification
Kathrin de la Rosa (Pieper) (EARLY CAREER SPEAKER)
11:50-12:05 Oral presentation 10+5’: Fc-Galactosylation of Human Immunoglobulin Gamma Isotypes Improves C1q Binding and Enhances Complement-Dependent Cytotoxicity
12:05-12:20 Oral presentation 10+5’: On-target hexamerisation of human IgG1 driven by a C-terminal fusion of a mutated IgM tail-piece for augmented complement activity
12:20-12:55 Bi-directional cross-talk between complement and IgG Fc-glycosylation controls autoimmune responses
13:00-16:20 Lunch & and free time
7: TECHNOLOGICAL ADVANCES
16:20-16:55 Antibody- and Complement-humanized mice
16:55-17:20 Oral presentation TECHNOLOGY 20+5’: DropMap – a technology for high-throughput single-cell deep phenotyping of immunoglobulin G secreting cells
Klaus Eyer (EARLY CAREER SPEAKER)
17:20-17:35 Oral presentation 10+5’: Characterizing autoantibody-producing cells in thrombocytopenic patients for secretion rate and affinity for a platelet autoantigen
Pablo Canales Herrerias
17:35-17:50 Oral presentation TECHNOLOGY 10+5’: Initiating the Classical Pathway: IgG recruitment, Hexamerization, and C1q binding in Real-Time
17:50-18:20 Coffee Break
18:20-18:45 In vivo analysis of anti-CD20 IgG induced B cell depletion in humanized mice (20+5’)
Anja Lux (EARLY CAREER SPEAKER)
18:45-19:05 IgG glycome composition is associated with biological age (15+5’)
Marija Pezer (EARLY CAREER SPEAKER)
19:05-20:00 Antibody repertoire technologies
20:15 Dinner & entertainment at the Hotel
8: Fc RECEPTORS I
09:00-09:35 Manipulating Fc receptors for improved immunotherapy
09:35-09:50 Oral presentation 10+5’: Accelerated clearance of cell free HIV by neutralizing antibodies is via an FcγRIIb-mediated effector function of liver sinusoidal endothelial cells
Latha Prabha Ganesan
09:50-10:05 Oral presentation 10+5’: Re-evaluating human IgG subclass efficacy for tumor clearance in vivo
10:05-10:40 IgG and FcyR play a pivotal role in intestinal immunity
10:40-10:55 Short Coffee Break
9: Fc RECEPTORS II
10:55-11:30 FcR-mediated anti-viral and anti-toxin effects
11:30-11:45 Oral presentation 10+5’: Testing human Fc-FcүR contributions to agonistic antibodies in a humanized mouse model system
Andrea Ipsen Escobedo
Closing Comments & PRIZES
11:45-11:55 Chairs Closing Comments & PRIZES
Pierre Bruhns & Falk Nimmerjahn
12:00 1st Bus Departure
15:00 PM 2nd Bus Departure
- Single (academic, industry) or double occupancy (students/post-docs) for 3 nights at the 4* hotel venue
- All meals & coffee breaks, drinks during poster sessions
- Guest registration includes accommodation (shared with the primary registrant), food and coffee breaks, but does not include participation in the workshop program
Abstracts are reviewed and selected by the workshop organizers based solely on the quality of content. Organizers may also select some abstracts for a short oral presentation at the workshop. All abstracts are assigned a poster number for presentation during a poster session at the meeting.
19 short-talks will be chosen from the selected abstracts by session chairs for oral presentations (7, 10 or 15 minutes depending on the session).
Exclude email addresses, phone numbers, hyperlinks, keyword lists, comments, text or image boxes.
2200 characters including spaces. Also includes title, authors, affiliations, references and funding.
One abstract per submitting author per meeting. Submitting author and account name must match. Submitting author must be underlined.
Each poster will have 1 m by 1 m (3.3 ft by 3.3 ft) of space available for display.
Thanks to the generosity of our sponsors (EMBO, EFIS, Antibodies, Complement Technology, Biolegend) 18 travel grants have been attributed to participants by the selection committee:
Travel Grant Awardees
|Title||First name||Last name||Country||Travel Grant||Financial Support|
|Ms.||Eleanor||Foy||United Kingdom||500 €||EFIS|
|Dr.||Latha Prabha||Ganesan||United States||500 €||Antibodies|
|Ms.||Andrea||Ipsen Escobedo||Germany||650 €||EFIS|
|Ms.||Rena||Liu||United Kingdom||500 €||EMBO|
|Ms.||Sofia||Macari||United Kingdom||500 €||EFIS|
|Ms.||Marina||Mané Damas||Netherlands||500 €||EFIS|
|Mr.||Michael||Marshall||United Kingdom||500 €||CompTech|
|Mr.||Kashyap||Patel||United States||500 €||Biolegend|
|Mr.||Joshua||Sopp||United Kingdom||650 €||EFIS|
|Mr.||Mark||Waterhouse||United Kingdom||650 €||EFIS|
Jury : Pr Galit Alter (USA), Pr Jose Caaveiro (Japan), Pr Menna Clatworthy (UK), Pr Mark Cragg (UK), Pr Fred Finkelmann (Israel),
Pr Andrea Tenner (USA).
Oral presentation Dr Mark Waterhouse (UK) 250€
Dr Klaus Eyer (France) 250€
Poster presentation Mr Georgios Delidakis (USA) 150€
Dr Guillaume Mottet (France) 150€
The organisers have enabled online credit card payments through PayPal. Please see the Payments section, above, for details.
Click below to begin online payment.
Punta Port Salvi s/n
Sant Feliu de Guixols
17220, Girona, Spain
Students/post-docs registration: 2 person room sharing
Academic/Industry registration: single room
Superior Double Rooms have terraces with sea view, bathroom, hair dryer, toilet, satellite-TV, minibar, safe, direct-dial telephone and central heating.
2 Restaurants: abundant barbecue buffet at noon and in the evenings a buffet with selected specialties of the region.
Comfortable lounges, elegant living rooms, 2 bars, bridge and billiards room.
The venue is located near Girona-Costa Brava Airport.
Transportation from Barcelona and Girona airports will be arranged if attendance to the meeting is sufficiently high (buses would be departing from both airports at times will we indicate closer to the meeting dates). We ask the confirmed participants to inform our secretary about their arrival airport and time.
In the event that buses can be arranged, the bus will be waiting on the ground floor of terminal 1, with a sign stating the name of the meeting. We cannot know in advance which platform the bus will be parked at, but the driver will be waiting for you.
ABOUT THE AREA
The Eden Roc Hotel is located only 1 km away from Sant Feliu de Guixols, in a unique and quiet peninsula. The clear Mediterranean sea surrounds the 360 meter wide Eden Roc Resort.